Purification and characterization of the diphtheria toxin repressor.
نویسندگان
چکیده
منابع مشابه
Metal stoichiometry and functional studies of the diphtheria toxin repressor.
Diphtheria toxin repressor (DtxR) is a transition metal ion-activated repressor in Corynebacterium diphtheriae. DtxR is an iron sensor; metal-bound DtxR represses transcription of genes downstream of the tox operator. Wild-type DtxR [DtxR(wt)] and several mutant forms were overexpressed and purified from Escherichia coli. DtxR was isolated without bound metal. Metal reconstitution gave a bindin...
متن کاملproduction and purification of polyclonal antibodies against diphtheria toxin
diphtheria is a fatal disease caused by exotoxin of corynebacterium diphtheria . this toxin consists of two chains, catalytic chain (a) and binding (b) chain. by binding chain (b), the toxin binds to its receptor on numerous body cells such as myocardial, kidney and peripheral nerve cells. after entering, catalytic chain (a) inhibits protein synthesis and finally can cause cell dea...
متن کاملCharacterization of high-order diphtheria toxin oligomers.
In 3D domain swapping, a domain of a protein breaks its noncovalent bonds with the protein core and its place is taken by the identical domain of another molecule, creating a strongly bound dimer or higher order oligomer. For some proteins, including diphtheria toxin, 3D domain swapping may affect protein function. To explore the molecular basis of 3D domain swapping in a well-characterized pro...
متن کاملCharacterization of a transferrin-diphtheria toxin conjugate.
We report here the synthesis and properties of a hybrid toxin prepared by covalently coupling diphtheria toxin to transferrin. The purified material contained two major hybrid protein species and was highly cytotoxic to mouse LMTK- cells in culture, reducing protein synthesis by 50% in 24 h at a concentration of 1 ng/ml. Cytotoxic activity was completely abolished in the presence of exogenous t...
متن کاملThe Purification and Concentration of Diphtheria Toxin: III. Separation of Toxin from Bacterial Protein.
Diphtheria toxin purified by a variety of methods has always contained bacterial protein. This raises the question whether the predominant protein in purified preparations is a fragment of the bacterial protoplasm which carries the toxin, or is itself the toxin. In a previous paper (Eaton, 1936b) it was shown that in various purified preparations there is no relation between the titer of bacter...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1992
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.89.16.7576